Download Coronaviruses: Methods and Protocols PDF Free

In this article, we are sharing with our audience the genuine PDF/ download of above mentioned Book/PDF/Document using direct links which can be found at the end of this blog post. To ensure user safety and faster downloads, we have uploaded this .pdf file to our online cloud repository so that you can enjoy a hassle-free downloading experience. Here at the Med-Dent, we believe in quality and speed which are a part of our core philosophy and promise to our readers. We hope that you people benefit from our blog! Now before we share the free PDF download with you, let’s take a look at a few of the important details regarding this ebook.

Download Coronaviruses: Methods and Protocols PDF Free

 

Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

1 Coronaviruses: An Overview of Their Replication and Pathogenesis . . . . . . . . 1
Anthony R. Fehrand Stanley Perlman
PART I DETECTION, DIAGNOSIS AND EVOLUTION OF CORONAVIRUSES
2 Identification of a Novel Coronavirus from Guinea Fowl
Using Metagenomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Mariette F. Ducatezand Jean-Luc Guérin
3 Serological Diagnosis of Feline Coronavirus Infection
by Immunochromatographic Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Tomomi Takanoand Tsutomu Hohdatsu
4 Estimation of Evolutionary Dynamics and Selection Pressure
in Coronaviruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Muhammad Munirand Martí Cortey
PART II PROPOGATION, TITRATION, AND PURIFICATION
OF CORONAVIRUSES
5 The Preparation of Chicken Tracheal Organ Cultures
for Virus Isolation, Propagation, and Titration . . . . . . . . . . . . . . . . . . . . . . . . 51
Ruth M. Hennion
6 The Preparation of Chicken Kidney Cell Cultures for Virus Propagation . . . . . 57
Ruth M. Hennionand Gillian Hill
7 Isolation and Propagation of Coronaviruses in Embryonated Eggs . . . . . . . . . 63
James S. Guy
8 Characterization of Human Coronaviruses on Well- Differentiated
Human Airway Epithelial Cell Cultures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Hulda R. Jonsdottirand Ronald Dijkman
9 Quantification of Infectious Bronchitis Coronavirus
by Titration In Vitro and In Ovo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Joeri Kint , Helena Jane Maier , and Erik Jagt
10 Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis. . . . 99
Stuart Dentand Benjamin W. Neuman
11 Partial Purification of IBV and Subsequent Isolation of Viral RNA
for Next-Generation Sequencing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Sarah M. Keep , Erica Bickerton , and Paul Britton
Contents

PART III MANIPULATING THE GENOMES OF CORONAVIRUSES
12 Transient Dominant Selection for the Modification and Generation
of Recombinant Infectious Bronchitis Coronaviruses. . . . . . . . . . . . . . . . . . . . 115
Sarah M. Keep , Erica Bickerton , and Paul Britton
13 Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial
Chromosomes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Fernando Almazán , Silvia Márquez-Jurado , Aitor Nogales ,
and Luis Enjuanes
PART IV CORONAVIRUS ATTACHMENT AND ENTRY
14 Protein Histochemistry Using Coronaviral Spike Proteins: Studying
Binding Profiles and Sialic Acid Requirements for Attachment to Tissues. . . . . 155
Iresha N. Ambepitiya Wickramasingheand M. Hélène Verheije
15 Identification of Protein Receptors for Coronaviruses
by Mass Spectrometry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
V. Stalin Raj , Mart M. Lamers , Saskia L. Smits , Jeroen A. A. Demmers ,
Huihui Mou , Berend-Jan Bosch , and Bart L. Haagmans
16 Single Particle Tracking Assay to Study Coronavirus Membrane Fusion. . . . . . 183
Deirdre A. Costelloand Susan Daniel
PART V STUDYING VIRUS-HOST INTERACTIONS
17 Studying Coronavirus–Host Protein Interactions. . . . . . . . . . . . . . . . . . . . . . . 197
Chee-Hing Yang , Hui-Chun Li , Cheng-Huei Hung , and Shih-Yen Lo
18 A Field-Proven Yeast Two-Hybrid Protocol Used to Identify
Coronavirus–Host Protein–Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . 213
Pierre- Olivier Vidalain , Yves Jacob , Marne C. Hagemeijer ,
Louis M. Jones , Grégory Neveu , Jean-Pierre Roussarie ,
Peter J. M. Rottier , Frédéric Tangy , and Cornelis A. M. de Haan
19 Investigation of the Functional Roles of Host Cell Proteins Involved
in Coronavirus Infection Using Highly Specific and Scalable RNA
Interference (RNAi) Approach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231
Jean Kaoru Milletand Béatrice Nal
20 Transcriptome Analysis of Feline Infectious Peritonitis Virus Infection. . . . . . . 241
Parvaneh Mehrbod , Mohammad Syamsul Reza Harun ,
Ahmad Naqib Shuid , and Abdul Rahman Omar
21 Quantification of Interferon Signaling in Avian Cells. . . . . . . . . . . . . . . . . . . . 251
Joeri Kintand Maria Forlenza
22 Studying the Dynamics of Coronavirus Replicative Structures . . . . . . . . . . . . . 261
Marne C. Hagemeijerand Cornelis A. M. de Haan
23 Preparation of Cultured Cells Using High-Pressure Freezing
and Freeze Substitution for Subsequent 2D or 3D Visualization
in the Transmission Electron Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Philippa C. Hawes

See also  Download Current Medical Diagnosis and Treatment 2021 60th Edition PDF Free

You might also be interested in:

Download Essentials of Family Medicine 6th Edition PDF Free

 

Product details

  • Hardcover: 300 pages
  • Publisher: Humana Press; 2015 edition (31 Mar. 2015)
  • Language: English
  • ISBN-10: 1493924370
  • ISBN-13: 978-1493924370

 

Download Coronaviruses: Methods and Protocols PDF Free

 

Alright, now in this part of the article, you will be able to access the free PDF download of Coronaviruses: Methods and Protocols PDF Free PDF using our direct links mentioned at the end of this article. We have uploaded a genuine PDF ebook copy of this book to our online file repository so that you can enjoy a blazing-fast and safe downloading experience.

Here’s the cover image preview of Coronaviruses: Methods and Protocols PDF Free:

File Size: 15.3 MB

 

 

Please use the direct link mentioned below to download Coronaviruses: Methods and Protocols PDF for free now:

 

Download Link

Disclaimer:
This site complies with DMCA Digital Copyright Laws.
Please bear in mind that we do not own copyrights to this book/software. We’re sharing this with our audience ONLY for educational purpose and we highly encourage our visitors to purchase original licensed software/Books. If someone with copyrights wants us to remove this software/Book, please contact us
. immediately.

You may send an email to [email protected] for all DMCA / Removal Requests.

Leave a Comment

error: